Summary of First Set of Microarray Data Displayed on AMPL

Microarray data were obtained from the NASCArrays website. All data were produced using the Affymetrix ATH1 Arabidopsis Genome Array. Only experiments using Columbia ecotype were selected. The y-axis of the bar graphs represents the "Signal" from Affymetrix chip. All data were normalized using the same method according to the NASC website "The top 2% and bottom 2% of signal intensities are excluded, then the mean is calculated. The original signal values are scaled such that the mean is made equal to 100." Warning statements are included on the bar graphs for genes with very close homologs in Arabidopsis such that probes are not gene specific. Additional information for each experiment can be obtained at the NASCArrays website. The following summary concerns data presented in bar graphs on the AMPL website.

Sd: Experiment of Miss Eleri Short. Control Col-0 seedlings grown for 2 weeks. RNA from whole plant. Data from 1 hybridization.

Oz: Experiment of Miss Eleri Short. Col-0 Seedlings grown for 2 weeks, (control is Sdl), fumigated with 500ppb ozone for 6 hrs. RNA from whole plant. Data from 1 hybridization.

SdC: Experiment of Dr Dorthe Villadsen. Control Col-0 seedlings grown for 1 week under constant light. RNA from whole plant. Data from 1 hybridization.

SdW: Experiment of Dr. Dorthe Villadsen. Control Col-0 seedlings grown for 1 week under constant light. Treated with water for 8 hours. RNA from whole plant. Data from 1 hybridization.

SdG: Experiment of Dr. Dorthe Villadsen. Col-0 seedlings grown for 1 week under constant light (controls are Sdl-C and Sdl-W). Treated with 30 mM glucose for 8 hours. RNA from whole plant. Data from 1 hybridization.

Rt: Experiment of Dr. Mark Jones. Primay roots from Col-0, grown on phytagel with 1% sucrose. Mean of 2 hybridizations.

LtC: Experiment of Dr. Peter Edward Unwin. Control Col-0 lateral roots from plants grown for 42 days. Data from 1 hybridization.

LtN: Experiment of Dr. Peter Edward Unwin. Col-0 lateral roots from plants grown for a total of 42 days (control is Lat-C). Harvested 21 days after infection with nematode Heterodera schachtii. Data from 1 hybridization.

ShW: Experiment of Irene Bramke. Control Col-0 shoots. Data from 1 hybridization.

ShC: Experiment of Irene Bramke. Shoots from Col-0 plants treated at 4C for 24 hours (control is Sh-W). Data from 1 hybridization

Pet: Experiment of Dr. Frank Millenaar. Petioles of Col-0. Data are mean from 2 hybridizations

AxA: Experiment of Dr. Sally Ward. Active axillary buds from Col-0 (axr1-12). Data from 1 hybridization.

AxD: Experiment of Dr. Sally Ward. Dormant axillary buds from Col-0 (axr3-1). Data from 1 hybridization.

Pol: Experiment of Dr. David Twell. RNA extracted from mature pollen of the Colombia ecotype. Data from 1 hybridization.

Susp: Experiment of Dr William Willats. Arabidopsis control suspension cells. Mean of three hybridizations.